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SRX22540334: GSM7903008: channel catfish heart, 10.8-month, replicate 3; Ictalurus punctatus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 24.5M spots, 7.3G bases, 2.4Gb downloads

External Id: GSM7903008_r1
Submitted by: Pathobiology, College of Veterinary Medicine, Auburn University
Study: Transcriptome analysis revealed potential mechanisms of channel catfish growth advantage over blue catfish in tank culture environment
show Abstracthide Abstract
Channel catfish and blue catfish represent two economically important freshwater aquaculture species in the United States. Our study aims to investigate the gene expression differences between these two catfish species by high-throughput RNA sequencing to understand their associated phenotypic differences in growth and disease resistant. Our transcriptomic analyses provide some insights into gene function differences between the two species and the molecular basis of channel catfish growth advantage in the tank culture environment. Overall design: Muscle, liver, intestine, mucus, and heart of channel catfish and blue catfish with three replicates were collected for RNA sequencing to identify the differentially expressed genes and tissue-specifically expressed genes.
Sample: channel catfish heart, 10.8-month, replicate 3
SAMN38271130 • SRS19549988 • All experiments • All runs
Library:
Name: GSM7903008
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA were extracted from muscle, liver, intestine, mucus, and heart of blue catfish and channel catfish with three replicates using Quick RNA Microprep Kit (Zymo Research, Irvine, CA, USA). The library for each tissue sample was constructed using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II RNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA)
Runs: 1 run, 24.5M spots, 7.3G bases, 2.4Gb
Run# of Spots# of BasesSizePublished
SRR2684478224,478,9857.3G2.4Gb2023-11-20

ID:
30534743

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